TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | endA hsdR supE thi-1 recA relA? gyrA φ80lacZΔM15 Δ(argF-lacZYA)U169 | Laboratory collection |
| JM109DE3 | endA recA gyrA thi hsdR17(rK− mK+) relA supE Δ(lac-proAB) [F′ traD36 proAB lacIq ZΔM15] | K. E. Sanderson |
| MV501 | VW187; wecA::Tn10 Tetr | 1 |
| VW187 | O7:K1; clinical isolate | 32 |
| Plasmids | ||
| pEX1 | Ampr ptac lacIqrrnBt | 26 |
| pKS | Bluescript II KS P, cloning vector; Ampr | 2 |
| pRL100 | 1.46-kb ClaI-XmaIII fragment containing wecA cloned in pBR322; Ampr | 22 |
| pUC18 | Cloning vector; Ampr | 36 |
| pAA8 | 41-bp fragment containing FLAG sequence in pUC18 HindIII site; Ampr | This work |
| pAA11 | 1.4-kb PCR fragment containing wecA cloned in pAA8; Ampr | This work |
| pAA12 | 1.4-kb fragment containing tagged wecA cloned in Bluescript II KS P; wecA+ Ampr | This work |
| pAA14 | 1.2-kb fragment containing part of wecA gene cloned in pEX1; wecA+ Ampr | This work |
| pAA15 | 0.98-kb fragment containing part of wecA gene cloned in pEX1; Ampr | This work |
| pAA16 | 0.76-kb fragment containing part of wecA gene cloned in pEX1; Ampr | This work |
| pAA19 | 1.5-kb fragment containing galF gene cloned in pAA8; Ampr | This work |