FIG 4.

Determination of subcellular localization of FtsZ in S. suis using the inducible expression system. (A) Determination of subcellular localization of FtsZ using pSSTete2-ftsZ_gfp. S. suis cells harboring pSSTete2-ftsZ_gfp were grown to the mid-log phase, followed by another 15-min incubation with or without ATc induction at 75 ng/mL. The cells were harvested, washed, stained with AF647 dye, and imaged using the NIS-Elements microscope (Nikon). (B) Determination of subcellular localization of FtsZ using pSSlace1-ftsZ_gfp. S. suis cells harboring pSSlace1-ftsZ_gfp were grown to the mid-log phase, followed by another 15-min incubation with or without IPTG induction at 0.2 mM. The cells were harvested, washed, stained with AF647 dye, and imaged using the NIS-Elements microscope (Nikon).