TABLE 1.

Optimal reaction conditions and primers used in this study

Assay	Target gene	Primer sequence	Concn (μM)	Significance
1	mgpa	MGpa_F, CTTGAGCCTTTCTAACCGCTGCACT	0.25	Species identification
		MGpa_R, CAAGTCCAAGGGGTTAAGGTTTCAT	0.25	Species identification
	HBB	HBB_F, AGTGCTCGGTGCCTTTAGTGAT	0.2	Quality control of nucleic acid extraction
		HBB_R, TGGCAAAGGTGCCCTTGA	0.2	Quality control of nucleic acid extraction
	parC	ParC_D87_F, CCCATGGTGATAGTTCCATTTAT	0.5	Supplementary test for distinguishing mutation S83N from D87N
		ParC_D87_R, AGCTTTGGGACATTCTGATAATTG	0.5	Supplementary test for distinguishing mutation S83N from D87N
2	parC	ParC_S8_F, GGGAGATCATGGGGAAATACC	0.0375	Prediction of fluoroquinolone resistance
		ParC_S83_R, CAGCTTTGGGACATTCTGATA	0.025	Prediction of fluoroquinolone resistance
		ParC_S83_P, CCCCCATGGTGATATTTCCATTTATDRTGCAAa	1	Prediction of fluoroquinolone resistance

^a3′-blocked oligonucleotide probe.