TABLE 2.
Tm values for all variants
| Assay | Target | PCR-amplicon type | Change |
Tm of: |
|||
|---|---|---|---|---|---|---|---|
| PCR-amplicon |
Probe-amplicon |
||||||
| Range | Mean ± SD | Range | Mean ± SD | ||||
| 1 | HBB | Quality control of nucleic acid extraction | 80.82–80.90 | 80.85 ± 0.024 | |||
| mgpa | Species identification | 75.44–75.67 | 75.60 ± 0.061 | ||||
| parC | D87WT | 70.85–70.83 | 70.77 ± 0.068 | ||||
| D87MT | 71.20–71.29 | 71.25 ± 0.027 | |||||
| 2 | parC | Type 1 | S83I (G248T) | 75.05–75.20 | 75.10 ± 0.042 | 64.57–64.66 | 64.61 ± 0.027 |
| S83N (G248A) | 75.03–75.13 | 75.10 ± 0.032 | 61.20–61.40 | 61.25 ± 0.008 | |||
| D87N (G259A) | 75.01–75.13 | 75.17 ± 0.039 | 60.88–61.16 | 61.04 ± 0.067 | |||
| D87Y (G259T) | 75.15–75.20 | 75.18 ± 0.022 | 60.24–60.39 | 60.32 ± 0.067 | |||
| Type 2 | WT | 75.57–75.71 | 75.60 ± 0.035 | 60.92–61.12 | 61.04 ± 0.056 | ||
| S83C (A247T) | 75.65–75.76 | 75.70 ± 0.034 | 59.32–59.55 | 59.41 ± 0.073 | |||
| D87H (G259C) | 75.53–75.65 | 75.60 ± 0.035 | 58.41–58.49 | 58.47 ± 0.037 | |||
| Type 3 | S83R (A247C) | 76.19–76.29 | 76.25 ± 0.030 | 59.36–59.52 | 59.42 ± 0.060 | ||
| D87G (A260G) | 76.16–76.26 | 76.23 ± 0.024 | 62.14–62.41 | 62.25 ± 0.067 | |||