TABLE 3.

Performance of the MGparC-AsyHRM method with 105 fully characterized M. genitalium clinical samples

AMR position (no. of samples)	Consistency with Sanger sequence method (%)	No. of samples that failed to provide valid data/total no. of samples (%)	No. of samples successfully producing valid data for different genomic copy ranges/total no. of samples (%) with:
			>2,000 copies/reaction		200–2,000 copies/reaction		20–200 copies/reaction		<20 copies/reaction
			Assay 1	Assay 2	Assay 1	Assay 2	Assay 1	Assay 2	Assay 1	Assay 2
WT (31)	28/29 (96.6)	2/31 (6.5)	3/3 (100)	3/3 (100)	5/5 (100)	5/5 (100)	18/18 (100)	17/18 (94.4)	5/5 (100)	4/5 (80)
S83I (50)	43/43 (100)	7/50 (14)	6/6 (100)	6/6 (100)	11/11 (100)	11/11 (100)	20/20 (100)	20/20 (100)	9/13 (69.2)	9/13 (69.2)
S83N (11)	10/10 (100)	1/11 (9.1)	1/1 (100)	1/1 (100)	1/1 (100)	1/1 (100)	4/4 (100)	4/4 (100)	4/5 (80)	4/5 (80)
S83R (1)	1/1 (100)	0/1 (0)	0/0 (100)	0/0 (100)	0/0 (100)	0/0 (100)	1/1 (100)	1/1 (100)	0/0 (100)	0/0 (100)
D87N (7)	5/6 (83.3)	1/7 (14.3)	0/0 (100)	0/0 (100)	0/0 (100)	0/0 (100)	3/3 (100)	3/3 (100)	2/3 (66.7)	2/3 (66.7)
D87Y (4)	3/3 (100)	1/4 (25)	0/0 (100)	0/0 (100)	1/1 (100)	1/1 (100)	2/2 (100)	2/2 (100)	0/1 (0)	0/1 (0)
Rare type (1)	1/1 (100)	0/1 (0)	0/0 (100)	0/0 (100)	1/1 (100)	1/1 (100)	0/0 (100)	0/0 (100)	0/0 (100)	0/0 (100)
Total (n = 105)	91/93 (97.8)	12/105 (11.4)	11/11 (100)	11/11 (100)	19/19 (100)	19/19 (100)	48/48 (100)	47/48 (97.9)	20/27 (74.1)	19/27 (70)