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The principle of the K. pneumoniae CRISPR-top assay. (a) Schematic illustration of the K. pneumoniae CRISPR-top workflow. The sample-to-answer time of the K. pneumoniae CRISPR-top assay is less than 90 min. (b) The principle of the CRISPR-top reaction. (c) The primer set (Kp1) and gRNA design regions. A part of the rcsA sequence (211–495 bp) was shown. Right-pointing arrows and left-pointing arrows indicate sense and complementary strands, respectively.
