TABLE 2.
Primers used for PCR amplification
| Primer | Sequence (5′→3′) | Function |
|---|---|---|
| cpsE-P1 | AAAACGACGGCCAGTGAATTCTGCTACAGCGGCACCAG | Construction of cpsE deletion mutant |
| cpsE-P2 | GCTCTTGTATAAGGTAAGGTTTGAAAGGAAT | |
| cpsE-P3 | ACCTTACCTTATACAAGAGCCCCTTACTTCCTT | |
| cpsE-P4 | CCGGGTACCGAGCTCGAATTCTACCACCAAATCCAACAGCAT | |
| cpsE-A | TCATCAACTGTGGGAGGG | Verification of cpsE deletion mutant |
| cpsE-B | TTGAAGCAATGGGAGTGA | |
| CcpsE-F | GAGCTCGGTACCCGGGGATCCTCCAACAGCATTACTTCAGA | Construction of cpsE complement strain |
| CcpsE-R | CAGGTCGACTCTAGAGGATCCGGCACCAGATGATATGATAAC | |
| RT-1F | CAGGTTTGTATGGCGTTGAG | Cotranscription analysis of cpsA-cpsD |
| RT-1R | TGCTGTTGGATTTGGTGGT | |
| RT-2F | CTCGTTTAGAAATAGTTGATA | Cotranscription analysis of cpsD-cpsE |
| RT-2R | ACCTTTAGTGTTAGGAGAATA | |
| RT-3F | GAACACGCCCTCCCACAG | Cotranscription analysis of cpsE-cpsI |
| RT-3R | ATGAGTCTGTTGTAGTACGGATTG | |
| RT-4F | TTGATTGGGTCAGACTGGATT | Cotranscription analysis of cpsH-cpsK |
| RT-4R | ATTCGTGGTCACTCACATTACA | |
| RT-5F | TGTAATGTGAGTGACCACGAAT | Cotranscription analysis of cpsK-cpsL |
| RT-5R | CCAAGCGAATAACCGAAAC | |
| RT-6F | GGGTCAAATACGGAGAAGAAT | Cotranscription analysis of cpsL-neuB |
| RT-6R | CCACAAGACACCGCAACAT | |
| RT-7F | AATGGTAGATGTTGCGGTGTC | Cotranscription analysis of neuB-neuA |
| RT-7R | CGCCGTTCGGATAGTATAAAG | |
| RT-8F | AATCAATCAATGGCTAACAG | Cotranscription analysis of neuA-ung |
| RT-8R | TTGAAGTGAAGGAGGAGC | |
| recA-F | ATTCAGGCGCAGTTGATTTAGTT | qRT-PCR for recA |
| recA-R | TCAATCTCAGCACGAGGAACA | |
| q-cpsA-F | GCAGGCTTTGGGCTTTGTTAG | qRT-PCR for cpsA |
| q-cpsA-R | GGACGTTTGGAGCTGTGAGGT | |
| q-cpsB-F | TTAGCACGTTCTTTACCGAAATCT | qRT-PCR for cpsB |
| q-cpsB-R | CCTTGATGTTAGACCGCCATT | |
| q-cpsC-F | GCAACAACTGAGACGAAGGATAA | qRT-PCR for cpsC |
| q-cpsC-R | ACGCCACAATATACTTCTTCAACA | |
| q-cpsE-F | TACAACGACACGACTTTC | qRT-PCR for cpsE |
| q-cpsE-R | ATCATAACAATCCTTTTCAG | |
| q-cpsJ-F | TCGCTATGTAATCAGAGGAAACTT | qRT-PCR for cpsJ |
| q-cpsJ-R | CGAATGGTGGGTTGTCAGAA | |
| q-neuA-F | CCAAATTGCCGGTAGAGCTATT | qRT-PCR for neuA |
| q-neuA-R | CGCAGTGGACGAATGTGTTAT | |
| Pro-cpsA-F | CAAATGAATTCCCGGGGATCCCCCATCTCAGTCTCAAGGTTC | Promoter of cpsA fused to pTCV-lac |
| Pro-cpsA-R | GTATCAACAAGCTGGGGATCCTGTAATTCTCCAATACTTAAAAAA | |
| Pro-aphA-3-F | CAAATGAATTCCCGGGGATCCCCCAGCGAACCATTTGAGG | Promoter of aphA-3 fused to pTCV-lac |
| Pro-aphA-3-R | GTATCAACAAGCTGGGGATCCCCGATTTTGAAACCAC | |
| 5′RACE-GSP | CGGACGTTTGGAGCTGTGAGGTAATGCTAT | 5′-RACE assay |
| 5′RACE-NGSP | CTCTAACAAAGCCCAAAGCCTGCTCAGA | |