FIG 2.

ΔccpA and a ΔcodY mutants have opposing effects on virulence factor expression. Cultures of WT LAC, ΔccpA, and ΔcodY were grown to midexponential phase (OD₆₆₀ = ~3–4), and samples were taken for RNA extraction. QRT-PCR was performed on these for expression of psmA normalized to rpoD (A). The same cultures were also grown overnight in TSB with (B) or without (C) glucose, and their supernatants were run on an SDS-PAGE gel for Western blot stained for α-hemolysin (B, C). Statistics: (A) unpaired t test. *, P < 0.05; **, P < .01; ****, P < .0001; (B) paired t test. *, P < .05; (C) unpaired t test. *, P < .05; ***, P < .001.