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Constitutive expression of AgrBD equalizes RNAIII::YFP stimulation across all mutants. Supernatants were removed from overnight cultures of WT LAC, ΔccpA, ΔcodY, and ΔagrA strains harboring plgt::agrBD. These were added to fresh cultures of WT LAC harboring a pYFP::RNAIII reporter plasmid. Averages of peak expression levels at hour 6 are shown in A, and a representative curve is shown in B. Statistics: mixed-effects model with Dunnett’s multiple-comparison test. ****, P < .0001.
