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. 2022 Jun 23;10(4):e01257-21. doi: 10.1128/spectrum.01257-21

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Copyright © 2022 Sudan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Relative gene expression of Toll-like receptors in IPEC-J2 cells. A coincubation assay was performed for 4 h with equal numbers of CP9 and ETEC in IPEC-J2 cells. The relative expression of TLR2 (A), TLR4 (B), and TLR9 (C) mRNAs was calculated by real-time PCR. The expression levels of all target genes were calculated relative to the housekeeping gene GAPDH using the 2^−ΔΔ^CT method. The values for the control cells were set to 1. The data are presented as means ± the SEM. Means marked with different letters (a, b, and c) differ significantly (P < 0.05).