TABLE 1.
Experimental setup and sample number for spiking experimenta
| Method code | Description | Water type | Water vol (mL) | Supernatant vol (mL) | Replicates (×) |
|---|---|---|---|---|---|
| A: PEG | Polyethylene glycol (PEG) precipitation | Distilled water | 20 50 200 |
15 37.5 150 |
3 3 3 |
| Distilled water—spiked | 20 50 200 |
15 37.5 150 |
3 2 3 |
||
| Wastewater | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| Wastewater—spiked | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| B: BE-PEG | Elution with beef extract (BE)—PEG precipitation | Distilled water | 20 50 200 |
15 37.5 150 |
3 3 3 |
| Distilled water—spiked | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| Wastewater | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| Wastewater—spiked | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| C: AS | Ammonium sulphate (AS) precipitation | Distilled water | 20 50 200 |
15 37.5 150 |
3 3 3 |
| Distilled water—spiked | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| Wastewater | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| Wastewater—spiked | 20 50 200 |
15 37.5 150 |
3 3 3 |
||
| D: AM | Amicon (AM) filtration | Distilled water | 20 50 |
15 20 |
3 3 |
| Distilled water—spiked | 20 50 |
15 20 |
3 3 |
||
| Wastewater | 20 50 |
15 20 |
3 3 |
||
| Wastewater—spiked | 20 50 |
15 20 |
3 3 |
||
| E: IP | InnovaPrep (IP) filtration | Distilled water—spiked | 20 50 |
15 37.5 |
3 3 |
| Wastewater—spiked | 20 50 |
15 37.5 |
3 3 |
a
Water volume is the volume of the sample taken and centrifuged to clarify the samples from solid matter. Supernatant volume refers to the volume of the clarified solution concentrated.