TABLE 3.
Comparison and ranking of the methods used for virus concentration in wastewatera
| Method | Viral recovery (%) | Sample vol (mL) | Concn factor (×) | Effective vol (mL) | Benchtop time/sample (min) | Overall time/sample (h) | Cost/sample (£) |
|---|---|---|---|---|---|---|---|
| PEG | 3.3 | 150 | 1500 | 6 | 50 | 18 | <1 |
| BE-PEG | 13.2 | 150 | 1500 | 6 | 60 | 18 | <1 |
| AS | 6.2 | 150 | 1500 | 6 | 40 | 3 | <1 |
| IP | 6.2 | 37.5 | 375 | 1.5 | 20 | 1 | 25 |
| Amicon | 14.1 | 15 | 150 | 0.6 | 30 | 1–2 | 8 |
a
The effective volume is the proportional volume of the original wastewater sample assayed by RT-qPCR (4 μL/reaction).