Fig. 2. Evaluation of the deaminase activity of A3A protein on cytosine, 4mC and 5mC by LC-MS/MS. Three synthesized 215-bp dsDNAs (DNA-C, DNA-4mC, and DNA-5mC) were used for the evaluation. (A) Extracted-ion chromatograms of the dC standard, dC from DNA-C without or with A3A treatment. (B) Extracted-ion chromatograms of the dU standard, dU from DNA-C without or with A3A treatment. (C) Extracted-ion chromatograms of 4mdC and 5mdC standards, 5mdC from DNA-5mC without or with A3A treatment. (D) Extracted-ion chromatograms of 4mdC and 5mdC standards, 4mdC from DNA-4mC without A3A or with A3A treatment.
