TABLE 2.
Virulence genes significantly different between HUS STEC and non-HUS STEC strains
| Gene | Function | No. of positive strains (%) |
P value | |
|---|---|---|---|---|
| HUS STEC (n = 52) | Non-HUS STEC (n = 188) | |||
| cdtA | Cytolethal distending toxin A | 29 (55.77) | 23 (12.23) | 4.05e−10a |
| cdtB | Cytolethal distending toxin B | 29 (55.77) | 23 (12.23) | 4.05e−10a |
| cdtC | Cytolethal distending toxin C | 29 (55.77) | 23 (12.23) | 4.05e−10a |
| espR4 | Type III secretion system effector EspR4 | 37 (71.15) | 82 (43.62) | 0.0005a |
| efa1 | EHEC factor for adherence Efa-1 | 33 (63.46) | 74 (39.36) | 0.0026 |
| astA | Heat-stable enterotoxin 1 EAST1 | 37 (71.15) | 92 (48.94) | 0.0047 |
| east1 | Enteroaggregative E. coli heat-stable enterotoxin 1 | 49 (94.23) | 147 (78.19) | 0.0077 |
| espFu | Type III secretion system effector EspFu | 7 (13.46) | 6 (3.19) | 0.0091 |
| clpV | Type VI secretion system ATPase ClpV | 49 (94.23) | 149 (79.26) | 0.0123 |
| espJ | Type III secretion system effector EspJ | 39 (75) | 105 (55.85) | 0.016 |
| espO1-2 | Type III secreted effector | 46 (88.46) | 136 (72.34) | 0.017 |
| espM1 | Type III secretion system effector EspM1 | 46 (88.46) | 136 (72.34) | 0.017 |
| chuV | ATP-binding hydrophilic protein ChuV | 41 (78.85) | 115 (61.17) | 0.0212 |
| chuW | Oxygen independent coproporphyrinogen III oxidase | 41 (78.85) | 115 (61.17) | 0.0212 |
| tssG | Type VI secretion system protein TssG | 46 (88.46) | 139 (73.94) | 0.0264 |
| nleB2 | Type III secretion system effector NleB2 | 41 (78.85) | 117 (62.23) | 0.0313 |
| shuS | Heme/hemoglobin transport protein ShuS | 40 (76.92) | 113 (60.11) | 0.0335 |
| shuA | Outer membrane hemoglobin receptor ShuA | 40 (76.92) | 113 (60.11) | 0.0335 |
| fha | Type VI secretion system protein Fha | 46 (88.46) | 140 (74.47) | 0.0384 |
| espL4 | Type III secretion system effector EspL4 | 43 (82.69) | 126 (67.02) | 0.0385 |
| tssF | Type VI secretion system protein TssF | 46 (88.46) | 141 (75) | 0.0391 |
| rhs | Type VI secretion system protein PAAR family | 35 (67.31) | 95 (50.53) | 0.0405 |
| aslA | Putative arylsulfatase | 41 (78.85) | 119 (63.3) | 0.0455 |
| espB | Type III secretion system protein EspB | 41 (78.85) | 119 (63.3) | 0.0455 |
| nleF | Type III secretion system effector NleF | 49 (94.23) | 156 (82.98) | 0.0458 |
| stcE | Metalloprotease StcE | 41 (78.85) | 120 (63.83) | 0.0460 |
| paa | Outer membrane adhesin Paa | 49 (94.23) | 155 (82.45) | 0.0462 |
| chuT | Periplasmic heme-binding protein ChuT | 2 (3.85) | 0 (0) | 0.0462 |
| chuU | Heme permease protein ChuU | 40 (76.92) | 115 (61.17) | 0.0485 |
| espP | Autotransporter serine protease EspP | 15 (28.85) | 110 (58.51) | 0.0002a |
| espR3 | Type III secretion system effector EspR3 | 10 (19.23) | 90 (47.87) | 0.0002a |
| ospG | Type III secretion system effector kinase OspG | 0 (0) | 33 (17.55) | 0.0004a |
| toxB | Cytotoxin ToxB | 13 (25) | 87 (46.28) | 0.0067 |
| cif | Type III secretion system effector Cif | 8 (15.38) | 60 (31.91) | 0.0231 |
| hcp1 | Type VI secretion system protein Hcp family | 11 (21.15) | 70 (37.23) | 0.0319 |
| nleA | Non-LEE-encoded effector NleA | 2 (3.85) | 29 (15.43) | 0.0333 |
| espX4 | Type III secretion system effector EspX4 | 48 (92.31) | 185 (98.4) | 0.0414 |
| entA | 23-dihydroxybenzoate-23-dehydrogenase Ent | 0 (0) | 15 (7.98) | 0.0461 |
a
Benjamini-Hochberg-corrected P < 0.05.