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Deletion of phage TT4P2-orf45 by the DPMSR method based on the heterologous CRISPR–Cas9 system. M is the DL5000 DNA Marker, and WT is the wild-type phage TT4P2. According to the PCR bands, plaques 3, 4, 7, 8 and 9 were recombinants, but the sequencing results revealed that they were heterozygous recombinants, which represented most of the recombinants. However, according to the sequencing results, plaques 1, 2, 5, 6 and 10 carried point mutations in the PAM.
