FIG 3.

WTA production affects the release of toxins from the S. aureus cell. (a and b) Western blotting detects toxin abundance in both intra- and extracellular extracts of the isogenic set of WTA-producing strains, demonstrating that some toxins are not released from the bacterial cells. (c and d) PSM production of the isogenic set of WTA-producing strains, demonstrating that some of the PSMs are not released from the bacterial cells. (e and f) Western blotting and PSM gels detect the abundance of Hla and the PSMs, showing that they are trapped within both the cell wall and the protoplast of the S. aureus cells. Full-length gels and Western blots with replicates can be found in Fig. S3 in the supplemental material.