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E6 variants (D32E and H85Y) drive enhanced degradation of p53. (A) E6-flag and GAPDH protein levels were assessed by Western blotting analysis. (B) CCK8 assay for exogenous HPV16 E6 expressing C-33A cells. (C, D) p53 and GAPDH protein levels were assessed by Western blotting analysis. GAPDH antibody was used as a loading control. *, P < 0.05; **, P < 0.01.
