TABLE 1.
Subtractive genomic analysis scheme toward the identification of novel therapeutic targets
| Serial no. | Steps/description | L. pneumophila subsp. pneumophilaa |
|---|---|---|
| 1 | Total no. of proteins | 2930 |
| 2 | No. of nonparalogous proteins (>60% identical) in CD-Hit | 2878 |
| 3 | No. of proteins nonhomologous to H. sapiens using BLASTp (E = 10−3) | 2010 |
| 4 | Essential proteins in DEG 15.2 server (E ≤ 10−100, bit score >100) | 125 |
| 5 | Essential proteins involved only in unique metabolic pathways (KAAS at KEGG) | 4 |
| 6 | Proteins assigned KO (KEGG orthology) but not assigned in any pathway | 29 |
| 7 | Hypothetical essential proteins assigned KO (KEGG orthology) but not assigned in any pathway | 3 |
| 8 | Essential membrane proteins using PSORTb, CELLO, ngLOC, PSLpred servers | 12 |
| 9 | Essential cytoplasmic proteins using PSORTb, CELLO, ngLOC, PSLpred servers | 13 |
| Protein of unknown location | 1 | |
| 10 | Essential proteins found to be novel in DrugBank 5.1.0 | 25 |
| 11 | Proteins nonhomologous to “anti-targets” using BLASTp (E < 0.005, identity <30%, query length >30%) | 25 |
| 12 | Membrane proteins showing antigenicity using VaxiJen v2.0 (threshold value >0.4) | 12 |
| 13 | No. of cytoplasmic and membrane proteins less similar to human microbiota using BLASTp against “43021[BioProject]” (E = 1, identity <47%) | 5 |
| 14 | Proteins selected for epitope design | 1 |
| 15 | Novel drug targets | 3 |
a
Strain Philadelphia 1/ATCC 33152/DSM 7513.