TABLE 8.
Allergenicity pattern of the predicted B-cell epitopes generated from LEGPH protein TolB
| Method | Start | End | Peptide | Length (aa)a | Allergenicity |
|---|---|---|---|---|---|
| BepiPred linear epitope prediction | 66 | 77 | SGPQGANSQSSV | 12 | Nonallergen |
| 404 | 417 | AREGDVQEPAWSPY | 14 | Allergen | |
| Chou and Fasman beta turn prediction | 194 | 200 | SWSPNGK | 7 | Nonallergen |
| 294 | 300 | SGRGGSP | 7 | Nonallergen | |
| Emini surface accessibility prediction | 206 | 212 | SFEKKKA | 7 | Nonallergen |
| 277 | 287 | IDTEPRYSPDG | 11 | Nonallergen | |
| Karplus and Schulz flexibility prediction | 160 | 166 | QRNGGRS | 7 | Allergen |
| 308 | 314 | ADGQISR | 7 | Nonallergen | |
| Kolaskar and Tongaonkar antigenicity scale | 5 | 23 | IISLFLLLFTGQVIALDLE | 19 | Nonallergen |
| 243 | 252 | GQHLAVVLSK | 10 | Nonallergen | |
| Parker hydrophilicity prediction | 174 | 180 | DADGHNP | 7 | Allergen |
| 283 | 289 | YSPDGRS | 7 | Nonallergen |
a
aa, amino acids.