Skip to main content
. 2022 Jul 12;10(4):e00373-22. doi: 10.1128/spectrum.00373-22

TABLE 9.

Allergenicity prediction and antigenicity analysis of designed vaccine constructs

Vaccine construct Composition Complete sequence of vaccine construct Allergenicity VaxiJen score (threshold 0.4)
V1 Predicted CTL, HTL, and BCL epitopes of LEGPH protein TolB with β defensin adjuvant and PADRE sequence EAAAKGIINTLQKYYCRVRGGRCAVLSCLPKEEQIGKCSTRGRKCCRRKKEAAAKAKFVAAWTLKAAAGGGSRIISLFLLLFTGGGSTSGRGGSPQVYGGGSQVIALDLELTQGGGSIGVQNTGGGPGGGSLPAREGDVQGGGSDVDLSSGSMKGGGSTFGNSIDTEGPGPGTPKIYDVDLSSGSMKQLTFGGPGPGLTFGNSIDTEPRYSPDGGPGPGFTSGRGGSPQVYRLSLAGPGPGYSLEVADADGHNPQSGPGPGRVTFEGNYNARASYTGPGPGGGRSRYSLEVADADGGPGPGISGPQGANSQSSVSTGPGPGVIALDLELTQGINSAKKSGPQGANSQSSVKKAREGDVQEPAWSPYKKSWSPNGKKKSGRGGSPKKSFEKKKAKKIDTEPRYSPDGKKADGQISRKKIISLFLLLFTGQVIALDLEKKGQHLAVVLSKKKYSPDGRSKKAKFVAAWTLKAAAGGGS Nonallergen 0.6034
V2 Predicted CTL, HTL, and BCL epitopes of LEGPH protein TolB with L7/L12 ribosomal protein adjuvant and PADRE sequence EAAAKMAKLSTDELLDAFKEMTLLELSDFVKKFEETFEVTAAAPVAVAAAGAAPAGAAVEAAEEQSEFDVILEAAGDKKIGVIKVVREIVSGLGLKEAKDLVDGAPKPLLEKVAKEAADEAKAKLEAAGATVTVKEAAAKAKFVAAWTLKAAAGGGSRIISLFLLLFTGGGSTSGRGGSPQVYGGGSQVIALDLELTQGGGSIGVQNTGGGPGGGSLPAREGDVQGGGSDVDLSSGSMKGGGSTFGNSIDTEGPGPGTPKIYDVDLSSGSMKQLTFGGPGPGLTFGNSIDTEPRYSPDGGPGPGFTSGRGGSPQVYRLSLAGPGPGYSLEVADADGHNPQSGPGPGRVTFEGNYNARASYTGPGPGGGRSRYSLEVADADGGPGPGISGPQGANSQSSVSTGPGPGVIALDLELTQGINSAKKSGPQGANSQSSVKKAREGDVQEPAWSPYKKSWSPNGKKKSGRGGSPKKSFEKKKAKKIDTEPRYSPDGKKADGQISRKKIISLFLLLFTGQVIALDLEKKGQHLAVVLSKKKYSPDGRSKKAKFVAAWTLKAAAGGGS Nonallergen 0.5524
V3 Predicted CTL, HTL, and BCL epitopes of LEGPH protein TolB with HABA adjuvant and PADRE sequence EAAAKMAENPNIDDLPAPLLAALGAADLALATVNDLIANLRERAEETRAETRTRVEERRARLTKFQEDLPEQFIELRDKFTTEELRKAAEGYLEAATNRYNELVERGEAALQRLRSQTAFEDASARAEGYVDQAVELTQEALGTVASQTRAVGERAAKLVGIELEAAAKAKFVAAWTLKAAAGGGSRIISLFLLLFTGGGSTSGRGGSPQVYGGGSQVIALDLELTQGGGSIGVQNTGGGPGGGSLPAREGDVQGGGSDVDLSSGSMKGGGSTFGNSIDTEGPGPGTPKIYDVDLSSGSMKQLTFGGPGPGLTFGNSIDTEPRYSPDGGPGPGFTSGRGGSPQVYRLSLAGPGPGYSLEVADADGHNPQSGPGPGRVTFEGNYNARASYTGPGPGGGRSRYSLEVADADGGPGPGISGPQGANSQSSVSTGPGPGVIALDLELTQGINSAKKSGPQGANSQSSVKKAREGDVQEPAWSPYKKSWSPNGKKKSGRGGSPKKSFEKKKAKKIDTEPRYSPDGKKADGQISRKKIISLFLLLFTGQVIALDLEKKGQHLAVVLSKKKYSPDGRSKKAKFVAAWTLKAAAGGGS Nonallergen 0.5726