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. 2022 Jul 5;10(4):e02327-21. doi: 10.1128/spectrum.02327-21

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Copyright © 2022 Kuang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 9 — Role of ROS in SCF-mediated killing. (A) Percent survival of AP-R_CLIN-EVO and AP-R_LAB-EVO in the presence of SCF (7.5 μg/mL) and the indicated concentrations of H₂O₂. (B) Percent survival of AP-R_CLIN-EVO and AP-R_LAB-EVO in the presence of H₂O₂ (0.6%) and the indicated concentrations of SCF. (C) Percent survival of AP-R_CLIN-EVO and AP-R_LAB-EVO in the presence of SCF (7.5 μg/mL) and H₂O₂ (0.6%) at the indicated times. (D) Percent survival of AP-R_CLIN-EVO and AP-R_LAB-EVO in the presence of H₂O₂ (0.6%), nitrite (20 mM), and SCF (7.5 μg/mL). (E) ROS of AP-R_CLIN-EVO and AP-R_LAB-EVO in the presence of the indicated H₂O_2. Results (A–D) were obtained using antibiotic bactericidal assay. Results are displayed as mean ± SEM of three biological replicas, and significant differences are identified (*, P < 0.05; **, P < 0.01) as determined by analysis of variance (ANOVA).