TABLE 1.

Results from three quantification methods (oil-enveloped bacteria, metal bath, and DNA kit) involving qPCR and ddPCR from a 10-fold serial dilution of V. cholerae strain C6706

Concentration of template	Oil-enveloped bacteria		Metal bath		DNA kit
	Cq mean or Copies/μL	SD	Cq mean or Copies/μL	SD	Cq mean or Copies/μL	SD
qPCR
13 ng/μL	12.16	0.06	13.42	0.07	13.89	0.02
1.3 ng/μL	15.29	0.2	16.84	0.14	17.40	0.06
130 pg/μL	18.91	0.05	21.62	0.32	20.86	0.15
13 pg/μL	22.48	0.76	25.78	0.53	24.63	0.34
1.3 pg/μL	26.05	0.52	29.28	0.36	27.83	0.6
130 fg/μL	29.37	0.16	31.67	0.37	30.98	0.37
13 fg/μL	33.63	0.25	35.4	0.95	33.78	0.3
R2	0.999		0.992		0.998
ddPCR
13 ng/μL	ULODa	ULOD	ULOD	ULOD	ULOD	ULOD
1.3 ng/μL	186000	7071.07	178000	5656.85	161900	8626.7
130 pg/μL	16900	2927.42	12050	2305.17	10730	1258.65
13 pg/μL	1240	466.69	1062	308.3	1450	523.26
1.3 pg/μL	186	49.50	86	5.66	180.5	6.36
130 fg/μL	24	9.89	18.1	3.54	25	9.9
13 fg/μL	3.5	2.40	3.6	1.70	3.3	1.56
R2	0.996		0.992		0.999

^aULOD, DNA concentration at which the signal of the assay was saturated (>20,000 copies in the reaction mixture).