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Assessment of the viability loss rate of V. cholerae strain C6706 on day 40 of VBNC-state development by quantification using oil-enveloped bacteria, a metal bath, and a DNA kit for qPCR and ddPCR
| Gene | Method | VBNC rate | Viability loss rate |
|---|---|---|---|
| Oil-enveloped bacteria | qPCR | 29.74% | 70.26% |
| ddPCR | 46.06% | 53.94% | |
| Metal bath | qPCR | 31.62% | 68.38% |
| ddPCR | 41.69% | 58.31% | |
| DNA kit | qPCR | 36.31% | 63.69% |
| ddPCR | 43.65% | 56.35% |
