TABLE 2.
Host | Lysogen | Promoter | β-Galactosidase activity
|
Slope (β-galactosidase vs μ)b | |
---|---|---|---|---|---|
Low μa | High μa | ||||
W1485 | JV2978 | wt | 373 | 767 | 0.68 |
JV2979 | C-1T | 755 | 777 | 0.02 | |
ΔBAGH | JV2978 | wt | 774 | 1,210 | 0.59 |
JV2979 | C-1T | 1,050 | 1,060 | 0.02 |
Growth rates (in doublings per hour) in MOPS (morpholinepropanesulfonic acid)-glycerol media with 0.4% glycerol (low growth rate) were 0.55 for W1485 and 0.49 for ΔBAGH. Growth rates in LB media (high growth rate) were 1.6 for W1485 and 1.3 for ΔBAGH.
Plots were scaled to 1.0 at a μ of 1.0 as follows. Growth rate slopes were determined by linear regression analysis. β-Galactosidase activities at a μ of 1.0 were then scaled to a value of 1.0. Absolute activities were divided by the scaling factor and replotted. The final slope was then determined by linear regression on the scaled values.