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. 2000 Jan;182(2):536–539. doi: 10.1128/jb.182.2.536-539.2000

TABLE 2.

Growth rate-dependent control still operates in strains with decreased rrn gene dosage

Host Lysogen Promoter β-Galactosidase activity
Slope (β-galactosidase vs μ)b
Low μa High μa
W1485 JV2978 wt 373 767 0.68
JV2979 C-1T 755 777 0.02
ΔBAGH JV2978 wt 774 1,210 0.59
JV2979 C-1T 1,050 1,060 0.02
a

Growth rates (in doublings per hour) in MOPS (morpholinepropanesulfonic acid)-glycerol media with 0.4% glycerol (low growth rate) were 0.55 for W1485 and 0.49 for ΔBAGH. Growth rates in LB media (high growth rate) were 1.6 for W1485 and 1.3 for ΔBAGH. 

b

Plots were scaled to 1.0 at a μ of 1.0 as follows. Growth rate slopes were determined by linear regression analysis. β-Galactosidase activities at a μ of 1.0 were then scaled to a value of 1.0. Absolute activities were divided by the scaling factor and replotted. The final slope was then determined by linear regression on the scaled values.