TABLE 2.
Primers used for SG-PERT, quantitative PCR, and semiquantitative PCR
| Gene | Sequence |
Technique | ||
|---|---|---|---|---|
| Forward primer | Reverse primer | Probe | ||
| MS2 phage PERT | TCCTGCTCAACTTCCTGTCGAG | CACAGGTCAAACCTCCTAGGAATG | SG-PERT | |
| MLV Env | CCTACTACGAAGGGGTTG | CACATGGTACCTGTAGGGGC | qPCR | |
| MLV IN | AAGCTCAGGCCAGGTAGAAAGAA | TCCCGATCTCCATTGGTTACCT | Revertant analysis | |
| Lmo2 | ACCGCTACTTCCTGAAAGCC | GACACCCACAGAGGTCACAG | qPCR | |
| Mecom2 | CGACAGAGGAGTGGGAGAAG | TCTAAGATCTTCCGATTTCTACGGC | qPCR | |
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG | qPCR | |
| Rodent GAPDH | TGTGTCCGTCGTGGATCTGA | CCTGCTTCACCACCTTCTTGA | FAM-CCGCCTGGAGAAACCTGCCAAGTATG-TAMRA | qPCR |