TABLE 3.
Primers used for amplification of integration sitesa
| Amplification step | Sequence |
|
|---|---|---|
| Oligonucleotide 1 | Oligonucleotide 2 | |
| PCR1 | ATTCGAAGCGAACAACACTGAC | CCTCTTGCAGTTGCATCCGAC |
| PCR2 | AATGATACGGCGACCACCGAGATCTACACCAGGACTGACGCTATGGTAATTGTTGACCCTTCGGATTACCCG | CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXXXAGTCAGTCAGCCTGATTGACTACCCGTCAGCGG |
X corresponds to nucleotide positions used as barcodes for sequencing. The set 1 oligonucleotides from PCR1 and PCR2 bind to the linker, while the set 2 oligonucleotides bind to the MLV LTR.