FIG 1.

Abundances of Pseudomonas on DNA and peptide level. (A) Phylogenetic tree based on 16S rRNA gene sequences of the most abundant Pseudomonas OTUs as well the isolate Hainich_002 that was used for necromass generation (star symbol). The tree was calculated using the arb neighbor-joining method (1000 bootstraps) within arb (81, 82). Closely related Pseudomonas species were added as references. (B) Relative abundances of Pseudomonas-related OTUs increase after necromass was added on day 0 and then progressively decrease throughout the incubation. Likely, also members of the genus Pseudomonas partake in the initial degradation of Pseudomonas-derived necromass. OTU00019, the most closely related OTU to the isolate Hainich_002 is highlighted in yellow. (C) Number of peptides associated with the genus Pseudomonas within the original groundwater at day 0, as well as the ¹²C labeled incubations over time. Unlike at the DNA level, most Pseudomonas-related peptides presumably stem from the added necromass.