TABLE 2.

Summary of serotyping results of serotype 14 variant (14-like) pneumococci from PNG

	Serotype resulta
	Molecular serotyping methods					Phenotypic serotyping methodse
Isolate	Microarrayb	PneumoCaT (% coverage)c	seroBA	SeroCall	Serotype 14 qPCR (ct value)d	Quellung	Latex agglutination
PMP1437	14-like	Nontypeable (44.50%)	14	Nontypeable	Negative (no ct)	Nontypeable	Nontypeable
PMP1438	14-like	Nontypeable (68.52%)	14	14	Positive (22.75)	Nontypeable	Nontypeable
PMP1514	14-like	Nontypeable (84.21%)	14	14	Positive (19.78)	Nontypeable	Nontypeable

^aThe number outside parentheses refers to the serotype call made by that method, e.g., ‘14’ indicates ‘serotype 14’.

^bSerotype by DNA microarray was initially determined from a swab following a culture amplification step and was subsequently repeated on the isolates derived from these samples to validate that the 14-like strains were isolated.

^cThe number inside parentheses represents the percent coverage against the serotype 14 cps locus, which was the top cps locus match for all three isolates.

^dThe number in parentheses represents the mean cycle threshold (ct) value obtained by quantitative PCR (qPCR) (wzy gene target) from duplicate wells.

^ePhenotypic serotyping was performed with all SSI pools and type 14 sera.