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. 2022 Jun 21;10(4):e00146-22. doi: 10.1128/spectrum.00146-22

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Copyright © 2022 Cheng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Virus release efficiency and Gag processing. (A) Western blotting (WB) analysis. 293T cells were transfected with WT pNL4-3 or p6^Gag mutants. At 24 h post-transfection, cell and virus lysates were collected and analyzed by WB with HIV immunoglobulin (HIV-Ig). Positions of HIV-1 Gag precursor Pr55^Gag, Gag processing intermediate p41 and HIV-1 capsid protein p24 are indicated. (B) Virus release efficiency. The relative efficiency of virus release was calculated as the amount of virion p24 divided by total Gag (virion p24 + cellular Pr55Gag + cellular p24). (C) Gag processing. Gag processing was expressed as the ratio of p24 relative to Pr55^Gag in virions. The data were plotted in bar graphs. The efficiency of virus release and Gag processing for WT was set as 100%. Error bars indicate the standard deviation (SD) from more than three independent experiments; ns, not significant. *, P < 0.05, **, P < 0.01, and ***, P < 0.001.