FIG 4.

Replication of HIV-1 WT and p6 mutants in Sup-T1 T cells (A), MT-4 T cells (B), and PBMCs (C and D). Both Sup-T1 and MT-4 T-cell lines were transfected with WT or mutant pNL4-3 molecular clones. Cells were split every day or every two days. Culture supernatants were collected for reverse transcriptase (RT) activity analysis. PBMCs from two donors were infected with virus stocks generated in 293T cells. Cells were split, and culture supernatants collected for RT activity analysis every 2-3 days. Virus replication was monitored by RT activity. Data in SupT1 and MT4 cell lines were obtained from two independent experiments and data in PBMCs cells were obtained only from one experiment.