FIG 5.
The role of the seven-amino-acid deletion in p6Gag and the overlapping deletion in p6*. Detection of cell- and virion-associated proteins by WB analysis. (A) 293T cells were co-transfected with HIV-1 proviral clones that encode Gag and GagPol at a ratio of 15:1. Two days post-transfection, virus and cell lysates were harvested and measured by WB. Virus release efficiency (B) and Gag processing (C) were calculated as described in Figure 3. Virus production for WT was set as 100%. Standard deviation was obtained from more than three independent experiments; ns, not significant. *, P < 0.05, **, P < 0.01, and ***, P < 0.001.