FIG 6.
The p6 mutants are resistant to inhibition by Alix V overexpression. (A) 293T cells were co-transfected with HIV-1 proviral DNA encoding WT Gag or the deletion of the 36th residue (ΔY), the 6-aa deletion (Δ6), or Δ7 mutant in p6Gag, together with control plasmid DNA (pcGNM2-Alix) or HA-Alix V or HA-Alix V/F676D expressing vector. At 24h post-transfection, cell-associated and virus-associated proteins were measured by WB. Pr55Gag, p41, p24 and HA-Alix V are indicated. The efficiency of virus release (B) and Gag processing (C) was calculated as described in Figure 3. The levels of WT virus produced in the absence of Alix V (-Alix) was set as 100%. Error bars show SD from four experiments. ns, not significant. *, P < 0.05, **, P < 0.01.