. 2022 Jun 21;10(4):e00146-22. doi: 10.1128/spectrum.00146-22

TABLE 5.

Phenotypes of HIV-1 wild-type and p6 mutant virions

Virus^a	Virus release^b		Maturation (p24/Pr55) %	Infectivity^b RLU^e (%)	Replication kinetics^f days of peak (% RT level)^g
Virus^a	RT^c (%)	VRE^d ⁽%)	Maturation (p24/Pr55) %	Infectivity^b RLU^e (%)	Sup-T1	MT-4	PBMC-D1	PBMC-D2
WT	100	100	100	100	11 (100.0)	4 (100.0)	7 (100)	9 (100)
ΔPTAP	15.9 ± 14.6	1.9 ± 1.4	65.0 ± 35.5		17 (31.3)	7 (29.4)	Defective	Defective
PΔ7	63.9 ± 26.1	62.1 ± 5.1	41.1 ± 12.1	69.1 ± 18.6	28 (14.8)	6 (64.1)	7 (39.9)	9 (16.0)
insPTAP	108.8 ± 25.3	113.4 ± 15.4	90.8 ± 26.7	113.5 ± 20.9	13 (116.8)	4 (132.4)	7 (96.7)	9 (85.0)
Δ7	73.0 ± 11.4	76.9 ± 11.0	47.0 ± 10.6	56.3 ± 3.6	21 (34.6)	6 (64.7)	7 (59.0)	10 (29.1)

WT, wild type; ΔPTAP, deletion of original PTAP motif; insPTAP, insertion of PTAPPE sequences; Δ7, deletion of 7-aa in p6; PΔ7, double mutation of insPTAP and Δ7.

The levels of p6 mutants were expressed as a percentage relative to WT level, which was arbitrarily set as 100%. Data were shown as mean value with standard deviation (SD).

RT, reverse transcriptase activity. Data are shown as mean ± SD.

VRE, virus release efficiency, is calculated as the amount of viron-p24 (CA) divided by the total amount of Gag (see Materials and Methods). Data are shown as mean ± SD.

RLU, relative luciferase unit.

Replication Kinetics, replication capacity of each mutant in T-cell lines (Sup-T1 and MT-4) or T-cells (PBMC Donor 1 and 2).

% RT level, the peak level of each mutant was normalized to WT, which is set as 100. All the data presented in Table 5 are summarized from Figure 3 and Figure 4.