FIG 2.

C. butyricum pretreatment attenuated the DSS-induced colonic barrier dysfunction. (A) Alican blue-PAS staining of the colon with its mucin secretion rate and immunohistochemical analysis of MUC2 with MUC2-stained coverage. Scale bar: 200 μm. (B) Immunofluorescence staining of tight junction protein Occludin expression in the colonic section from mice (n = 5). Blue: DAPI; Green: Occludin. (C to E) Relative mRNA levels of Mucin secretion proteins, tight junction proteins, and proinflammatory related gene expression (n = 8). *, P ≤ 0.05; **, P ≤ 0.01 versus the control group, ^#, P ≤ 0.05; ^##, P ≤ 0.01 versus the DSS group.