FIG. 5.

RT-PCR analysis. Cells of strains UQ285 [rpoD(Ts) rpoS⁺] and AJW1404 [rpoD(Ts) rpoS::Km] were subjected to the temperature shift protocol described in the legend to Fig. 1. Lanes 1 to 6, strain UQ285; lanes 7 to 12, strain AJW1404; lanes 1, 2, 7, and 8; cells grown at 32°C and harvested during mid-exponential growth just prior to dividing the culture; lanes 3, 4, 9, and 10, cells further incubated at 32°C and harvested upon entry into stationary phase; lanes 5, 6, 11, and 12, cells further incubated at 42°C and harvested at the same time as those incubated at 32°C; lanes 1, 3, 5, 7, 9, 11, primer pair F5 and R1, which amplifies a 563-bp region, was used (see Fig. 3); lanes 2, 4, 6, 8, 10, and 12, primer pair F6 and R1, which amplifies a 460-bp region, was used (see Fig. 3).