TABLE 1.

Forward primer, reverse primer, and probes of the Del96/70, E484A and N501Y NATs

Mutation	Primer/probe	Sequence (5′→3′)	Positiona
Del69/70	Forward primer	CTTACCTTTCTTTTCC	21727–21742
	Reverse primer	GGTTATCAAACCTCTTAG	21806–21789
	Probe wild type	GGTTCCATGCTATACA	21753–21768
	Probe mutant-version1	GTTCCATGCTATCTCT	21754–21768
	Probe mutant-version 2	GGTTCCATGTTATCTCT	21753–21768
E484A	Forward primer	ACTGAAATCTATCAGG	22970–22985
	Reverse primer- version1	TGTAAAGGAAAGTAAC	23040–23025
	Reverse primer- version2	CGTAAAGGAAAGTAAC	23040–23025
	Probe wild type	TGTAATGGTGTTGAA	23000–23014
	Probe mutant	TTGTAATGGTGTTGCA	23001–23014
N501Y	Forward primer- version1	AAGGTTTTAATTGTTACTTTCC	23013–23034
	Forward primer- version2	CAGGTTTTAATTGTTACTTTCC	23013–23034
	Reverse primer	GAAGTTCAAAAGAAAGTACTAC	23114–23093
	Probe wild type	TTCCAACCCACTAATGG	23051–23067
	Probe mutant-version1	TTCCAACCCACTTATGG	23051–23067
	Probe mutant-version2	TTCCGACCCACTTATGG	23051–23067

^aPositions according to SARS-CoV-2 isolate Wuhan-Hu-1 (acc. no. NC_045512.2).