FIG 8.

Colocalization of nsp13 with other replicase proteins and dsRNA in SARS-CoV-2-infected cells. (A) A549-ACE2 cells were infected with SARS-CoV-2 (MOI = 2), fixed at 6-h postinfection (hpi), and costained with rabbit anti-SARS nsp13 pAb and appropriate home-made mouse anti-SARS-CoV-2 nsp sera or mouse anti-dsRNA MAb, followed by staining with Alexa Fluor 488-conjugated goat anti-rabbit Ab (green) and Alexa Fluor 555-conjugated goat anti-mouse Ab (red). Cell nuclei were stained with DAPI (blue) and examined by confocal microscopy. Images in the fifth column were obtained at higher magnification to show single-cell details of fluorescence labeling; scale bars represent 10 μm. The intensity distribution describes the colocalization of nsp13 with other replicase proteins for specific fluorescence along the indicated line. (B) Pearson’s correlation analysis demonstrated colocalization of nsp13 with other replicase proteins. One-way analysis of variance (ANOVA) was used for multiple comparisons on the colocalization between nsp13 and different nsps in GraphPad Prism 8.4.3 software. ****, P < 0.0001; ***, P < 0.001.