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Interaction of nsp8 with other replicase proteins. HEK-293T cells were transfected with equal amounts (2 μg) of the empty vector pRK5-Flag alone (as a control), pRK5-nsp8-Flag, and pRK5-nsp7 (A)/nsp8-Myc (B). Whole-cell lysates (WCLs) were pre-adsorbed onto Dynabeads protein G and incubated with anti-Flag antibodies for coimmunoprecipitation analysis. The protein complex was detected by a horseradish peroxidase (HRP)-conjugated anti-Flag MAb and HRP-conjugated anti-Myc MAb.
