TABLE 1.
Bacterial strains used in this study
| Strain | Genotype or phenotypea | Source or referenceb |
|---|---|---|
| B. subtilis | ||
| WN355 | metC14 sul thyA1 thyB1 trpC2 uvrB42 ΔsplB::ermC | 19 |
| WN356 | metC14 sul thyA1 thyB1 trpC2 uvrB42 ΔsplAB::ermC | 19 |
| WN443 | As for WN355, Δ3 splB-lacZ at amyE | pWN419→WN355 |
| WN444 | As for WN355, Δ4 splB-lacZ at amyE | pWN420→WN355 |
| WN446 | As for WN355, down −35 P1 splB-lacZ at amyE | pWN425→WN355 |
| WN447 | As for WN355, up −35 P1 splB-lacZ at amyE | pWN426→WN355 |
| WN448 | As for WN355, ΔsplA,splB-lacZ at amyE | pWN428→WN355 |
| WN450 | As for WN356, Δ3 splB-lacZ at amyE | pWN419→WN356 |
| WN451 | As for WN356, Δ4 splB-lacZ at amyE | pWN420→WN356 |
| WN453 | As for WN356, down −35 P1 splB-lacZ at amyE | pWN425→WN356 |
| WN454 | As for WN356, up −35 P1 splB-lacZ at amyE | pWN426→WN356 |
| WN455 | As for WN356, ΔsplA,splB-lacZ at amyE | pWN428→WN356 |
| WN459 | As for WN356, Δ2 splAB-lacZ at amyE | pWN418→WN356 |
| WN465 | As for WN355, Δ2 splAB-lacZ at amyE | pWN418→WN355 |
| WN491 | As for WN459 (pMK3) | pMK3→WN459 |
| WN492 | As for WN459 (pWN489) | pWN489→WN459 |
| WN520 | As for WN450 (pMK3) | pMK3→WN450 |
| WN521 | As for WN453 (pMK3) | pMK3→WN453 |
| WN522 | As for WN455 (pMK3) | pMK3→WN455 |
| WN523 | As for WN454 (pMK3) | pMK3→WN454 |
| WN525 | As WN450 (pWN489) | pWN489→WN450 |
| WN526 | As WN453 (pWN489) | pWN489→WN453 |
| WN527 | As WN454 (pWN489) | pWN489→WN454 |
| WN528 | As WN455 (pWN489) | pWN489→WN455 |
| E. coli JM109 | endA1 recA1 gyrA96 thi hsdR17 (rK− mK+) relA1 supE44 λ− Δ(lac-proAB) [F′ traD36 proA+B+laclqZΔM15] | Laboratory stock (31) |
a
Plasmids in parentheses are extrachromosomal.
b
Arrows denote introduction by transformation.