TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pMK3 | E. coli-B. subtilis shuttle vector | 28 |
| ptrpBG-1 | lacZ fusion vector, integrates at amyE | 27 |
| pUC18 | Multisite E. coli cloning vector | 31 |
| pWN41 | 2.3-kb EcoRI-HindIII splAB-containing fragment cloned in pBGSC-6 | 23 |
| pWN418 | Wild-type Δ2 splAB-lacZ in ptrpBG-1 | This study |
| pWN419 | Δ3 splB-lacZ in ptrpBG-1 | This study |
| pWN420 | Δ4 splB-lacZ in ptrpBG-1 | This study |
| pWN425 | Down −35 P1 splB-lacZ in ptrpBG-1 | This study |
| pWN426 | Up −35 P1 splB-lacZ in ptrpBG-1 | This study |
| pWN428 | ΔsplA,splB-lacZ in ptrpBG-1 | This study |
| pWN489 | pMK3 carrying the splA cistron | This study |