TABLE 1.
Features of the As389/HHQ-BSA ELISA for the detection of HQNO
| Metric | PBSTa | MH | MH diluted 1/5 |
|---|---|---|---|
| Amin | 0.09 ± 0.04 | 0.12 ± 0.01 | 0.09 ± 0.02 |
| Amax | 1.08 ± 0.06 | 1.32 ± 0.08 | 0.85 ± 0.01 |
| Slope | −0.75 ± 0.04 | −0.64 ± 0.03 | −0.72 ± 0.06 |
| IC50 | 4.20 ± 0.86 | 14.65 ± 2.21 | 2.71 ± 0.04 13.55 ± 0.20) |
| Dynamic range | 0.72 ± 0.18 to 26.71 ± 0.96 | 1.84 ± 0.38 to 105.37 ± 5.98 | 0.41 ± 0.10 to 17.04 ± 1.08 |
| LOD | 0.27 ± 0.09 | 0.60 ± 0.13 | 0.15 ± 0.05 (0.75 ± 0.25) |
| R2 | 0.987 ± 0.013 | 0.998 ± 0.001 | 0.990 ± 0.004 |
a
The concentration of BSA conjugate and dilution used in the assay run in buffer (PBS-6.5) was 0.25 μg mL−1 and 1/8000, respectively. In the case of the assay run in MH or MH diluted 1/5 the concentration of BSA conjugate and As dilution were 0.25 μg mL−1 and 1/8000, respectively. The parameters and features of the MH 1/5 curve correspond and refer to the values in the diluted sample and brackets are calculated for the corresponding IC50 and LOD in MH culture media. The concentrations are expressed in nM and the data shown correspond to the average of 3 different days using at least 2 well/replicates per concentration.