TABLE 2.
Half maximal inhibitory concentration (IC50) of the As389/HHQ-BSA ELISA using as analytes HHQ, PQS, HQNO, PYO, IQS, and the quinolone-type antibiotics ciprofloxacin and norfloxacin
| Quinolone | IC50a | C.R.b (%) |
|---|---|---|
| HHQ | 56.0 | 19 |
| PQS | 162.5 | 7 |
| HQNO | 10.7 | 100 |
| PYO | - | <0.01% |
| IQS | - | <0.01% |
| DHQ | - | <0.01% |
| 2-AA | - | <0.01% |
| Ciprofloxacin | - | <0.01% |
| Norfloxacin | - | <0.01% |
Dashes indicates that there is no cross-reactivity when testing that combination.
The percentages of cross-reactivity (C.R.) were calculated following the equation: CR (%) = IC50(cross reactant)/IC50(analyte) × 100.