TABLE 3.

Coefficients of variation (CV) of the As389/HHQ-BSA ELISA run in MH culture broth diluted 1/5 using representative concentrations at a low, medium, and high concentration range (IC₂₀, IC₅₀, and IC₈₀)

Reproducibility conditions	IC	Mean	Desv. est.	% CVa
Interday	20	26.71	3.36	12.6
	50	4.32	0.89	20.6
	80	0.73	0.26	36.2
Interplate	20	25.72	3.91	15.2
	50	2.87	0.56	19.5
	80	0.32	0.12	37.5
Intraplate	20	24.34	3.35	13.8
	50	2.75	0.27	9,9
	80	0.29	0.04	13.9

^aThe coefficient of variation (CV) was calculated following the equation CV (%) = σ/μ × 100. The results were obtained by measurements performed in either triplicate on the same ELISA plate (intraplate), made on three different days (interday), or by analysis on three different plates (interplate). The concentrations of the replicates, mean, standard deviation, and ICs are expressed in nM. IC: inhibitory concentration; R: replicate; σ: standard deviation; μ: Average.