S. aureus core genome phylogeny colored by geographical origin of isolation (strain name color) and MLST (middle bar) and clade (right bar). S. aureus isolates were from different parts of the region and clustered into six clades, each containing Egyptian isolates. Clade 1 isolates belonged to ST-1 and were from Egypt and the United Arab Emirates, and clade 2 contained the majority of the Arab isolates, with spa t044/SCCmec IV/ST-80 as the predominating clone. Clade 3 isolates were solely from Egypt and belonged mainly to ST-15 and ST-5. Clade 4 comprised isolates from Egypt, Sudan, and Palestine, with the majority belonging to ST-22 and ST-361. Clade 5 contained isolates from Egypt and belonged mainly to ST-97. The remaining isolates were in clade 6, of ST-239, and from Egypt and Morocco (n = 1). This clade represents a spa t037/SCCmec III/MLST CC8 clone.