FIG 3.

SsARO8 is involved in tryptophan catabolism. (A) Contents for tryptophan (Trp), l-kyrunine (l-Kyn), tryptamine (TPA), and IAA of the wild-type (WT) or ssaro8Δ sporidia, cultured under control (untreated) or Trp-supplemented (+Trp) conditions, were quantified by tryptophan metabolomics analysis (details in Materials and Methods) and presented in boxplots. Different letters indicated significant difference (P < 0.05, n = 6). (B) Detection of intracellular TryOH content from the WT or ssaro8Δ sporidia cultured under the same condition as that described in panel A. Fifty micromolar TryOH (Sigma, 188255) solution served as a standard. Left panel shows the HPLC chromatograms (10 μM injection) of TryOH (standard) or fungal extract(s). RT, retention time. Right panel, MS/MS spectra of the peak (RT = 2.64 min) in TryOH (standard) and WT+Trp sample, obtained by isolation of the protonated molecular ion (m/z 162.09). Arrows denote the peaks corresponding to TryOH.