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. 2022 Jul 12;10(4):e01050-22. doi: 10.1128/spectrum.01050-22

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Copyright © 2022 Xu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Production and identification of the rE2 protein in rice endosperm. (A) Genetic transformation of rice includes rice cultivation, callus induction, root induction, harvest of intact plants, and collection of mature transgenic rice. (B) Screening of rice plants expressing rE2 by dot blot analysis. Dots 1 to 8 are eight different kinds of transgenic plants. Dot 9 is a TP309 nontransgenic plant (TP309). (C) SDS-PAGE (left) and Western blotting (right) analysis of the purified rE2 protein. M, protein marker. Columns 1 and 2, wash-off fractions. The red arrowhead indicates the rE2 protein.