FIG 3.

Specificity and sensitivity of rE2-based ICTSs. (A) Specificity of the ICTSs. Seven different serum samples with pathogenic pig viruses were evaluated. No cross-reactivity was detected with the ICTSs. (B) Reactivity of rE2 with CSFV-positive serum and BVDV-positive serum was measured by ELISA (upper) and dot blotting (lower). CSFV+, CSFV-positive serum; BVDV+, BVDV-positive serum; NC, the extraction of soluble protein from TP309. (C) Sensitivity of the rE2-based ICTSs. The CSFV-positive serum was diluted from 1:1,000 to 1:256,000 and tested. C, control line; T, test line. (D) Relative optical density curves of the chromogenic results for the rE2-based ICTSs. (E) Standard curve between the G/D × A value and dilution of E2-positive serum.