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Structure of the rE2-based ICTSs and result readout. (A) For the CSFV-positive serum samples, the gold-rE2 conjugate forms a complex with anti-E2 IgG before binding to SPA, which combines with the IgG Fc domain of the antigen-Ab complex. As a result, the T line with SPA turns red. (B) For a negative serum sample, serum IgG is only combined with anti-pig IgG on the control line, with no color on the test line. C, control line, anti-pig IgG; T, test line, SPA.
