TABLE 1.
Optimal conditions for the ICTSs
| Condition | Result |
|---|---|
| Protein buffer | PBS |
| Protein concn | 6 μg/mL |
| Optimal pH | 7.5–8.0 |
| Vol of colloidal gold-labeled rE2 | 6 μL/cm |
| Concn of SPA | 0.5 mg/mL |
| Concn of anti-IgG | 1 mg/mL |
Optimal conditions for the ICTSs
| Condition | Result |
|---|---|
| Protein buffer | PBS |
| Protein concn | 6 μg/mL |
| Optimal pH | 7.5–8.0 |
| Vol of colloidal gold-labeled rE2 | 6 μL/cm |
| Concn of SPA | 0.5 mg/mL |
| Concn of anti-IgG | 1 mg/mL |