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. 2022 Jul 12;10(4):e01050-22. doi: 10.1128/spectrum.01050-22

TABLE 1.

Optimal conditions for the ICTSs

Condition Result
Protein buffer PBS
Protein concn 6 μg/mL
Optimal pH 7.5–8.0
Vol of colloidal gold-labeled rE2 6 μL/cm
Concn of SPA 0.5 mg/mL
Concn of anti-IgG 1 mg/mL